AdipoGen Life Sciences

SARS-CoV-2 Neutralizing Antibodies Detection Kit (B.1.351 Variant, Beta)

CHF 840.00
In stock
AG-48B-0005-KI0196 wellsCHF 840.00
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Product Details
Synonyms SARS-CoV-2 Neutralizing Antibodies Detection Kit (B.1.351 Variant, South Africa); COVID-19 Neutralizing Antibodies Detection Kit (PB.1.351 Variant, Beta); 2019-nCoV Spike Protein S1 (RBD) Neutralizing Antibodies Detection Kit (B.1.351 Variant, Beta)
Product Type Kit
Properties
Application Set Qualitative ELISA
Specificity

To measure the presence of neutralizing/blocking antibodies in human serum/plasma that inhibit the binding of the SARS-CoV-2 Spike (RBD) (B.1.351 Variant, Beta) protein to its human receptor ACE2.

Crossreactivity Avian
Bovine
Goat
Guinea pig
Human
Monkey
Mouse
Pig
Rabbit
Rat
Quantity

Contains sufficient materials for 96 reactions

Sample Type Plasma
Serum
Detection Type Colorimetric
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Plate and reagents should reach room temperature before use.
Use/Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.
Documents
Manual Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Coronaviruses (CoVs) are enveloped non-segmented positive-sense single-stranded RNA viruses and can infect respiratory, gastrointestinal, hepatic and central nervous system of human and many wild animals. Recently, a new severe acute respiratory syndrome β-coronavirus called SARS-CoV-2 has emerged, which causes an epidemic of acute respiratory syndrome (called coronavirus human disease 2019 or COVID-19). SARS-CoV-2 contains 4 structural proteins, including Envelope (E), Membrane (M), Nucleocapsid (N) and Spike (S), which is a transmembrane protein, composed of two subunits S1 and S2. The S1 subunit contains a receptor binding domain (RBD), which binds to the cell surface receptor Angiotensin-Converting Enzyme 2 (ACE2) present at the surface of epithelial cells, causing mainly infection of human respiratory cells.The SARS-CoV-2 Neutralizing Antibodies Detection Kit (B.1.351 Variant, Beta) contains key reagents required to test the presence of functional neutralizing antibodies against SARS-CoV-2 (variant B.1.351) present in the serum or plasma or/and to test the cross-reaction of SARS-CoV-2 neutralizing antibodies directed to previous variants. It is an easy and fast alternative to the classical neutralization assay using Vero E6 cells. This Detection Kit is based on a colorimetric reaction, which measures the binding of the RBD of the Spike S protein from SARS-CoV-2 to its human receptor ACE2. The presence of neutralizing/blocking antibodies in the samples are detected by reduction of signal indicating the inhibition of the Spike-ACE2 binding. 

The new variant of SARS-CoV-2, called B.1.351 (Beta), was detected in South Africa. This variant carries three mutations in the RBD at the positions 417, 484 and 501 (K417N, E484K, N501Y) and is associated with a higher viral load, which may suggest potential for increased transmissibility.

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